Fig. 3

CCNB2 was predicted and verified as a direct target of miR-582–3p. a A schematic of the bioinformatics prediction for the 3′-UTR of CCNB2 as well as for the mutant 3′-UTR used here. b In the dual-luciferase assay, THP-1 cells were co-transfected with miR-582–3p mimics or miR-NC and CCNB2 plasmid with wild-type or mutant 3′-UTR. Firefly luciferase activity was normalized to Renilla luciferase activity. Data are presented as means ± SD from three independent experiments. **p < 0.01 vs. miR-NC; c The protein level of cyclin B2 expression in miR-582–3p-overexpressing cells was determined using western blot. d The expression of CCNB2 mRNA was determined in fresh blood samples from patients with AML and healthy controls (n = 20). e The protein expression of cyclin B2 was determined in 3 leukemia cell lines (THP-1, Molt-4 and K562) and normal HS-5 cells using western blot. ***p < 0.001 vs. miR-NC; ^##p < 0.01, ^###p < 0.001 vs. HS-5