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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Down-regulation of miR-30b-5p protects cardiomyocytes against hypoxia-induced injury by targeting Aven

Fig. 4

miR-30b-5p targets the 3′-UTR of Aven. a Sequence alignment of miR-30b-5p and 3′-UTR of Aven. b Dual-luciferase reporter assay. AC16 cells were co-transfected with miR-30b-5p inhibitor and a luciferase reporter containing the Aven 3′-UTR or mutant Aven 3′-UTR and incubated for 48 h. Relative luciferase activities were detected by the dual luciferase assay system. ***p < 0.001 vs. NC. The mRNA (c) and protein (d) expression levels of Aven were detected by qRT-PCR and Western blot analysis, respectively. *: hypoxia vs. normoxia; #: hypoxia + inhibitor vs. hypoxia + NC; ***p < 0.001, ##p < 0.01, ###p < 0.001

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