Fig. 2

MiR-376c-3p attenuated OGD-induced cell injury. PC-12 and SH-SY5Y cells were transfected with miR-376c-3p mimic or miR-NC and then subjected to OGD. (a) Quantitative reverse transcription PCR analysis of miR-376c-3p expression in PC-12 and SH-SY5Y cells. (b) Cell viability was measured using the CCK-8 assay. (c) Cell cycle distribution was analyzed via flow cytometry with PI staining. (d) Cell apoptosis was examined using flow cytometry with Annexin V/PI double staining. (e) The expression of miR-376c-3p was analyzed in PC-12 cells after transfection with anti-miR-376c-3p or anti-miR-NC. (f) Cell viability was measured in PC-12 cells. (g) Cell cycle distribution was analyzed using flow cytometry with PI staining in PC-12 cells. (h) Cell apoptosis was examined using flow cytometry with Annexin V/PI double staining in PC-12 cells. Data are expressed as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. miR-NC; ^##p < 0.01, ^###p < 0.001 vs. anti-miR