Fig. 3
From: MiR-214 prevents the progression of diffuse large B-cell lymphoma by targeting PD-L1
The regulatory relationship between miR-214 and PD-L1. (a) The bioinformatics analysis showed that miR-214 has a binding site with PD-L1. (b) The dual-luciferase reporter gene assay was used to verify the targeted relationship between miR-214 and PD-L1. (c and d) The expression of PD-L1 protein was determined using western blot. (e) Quantitative RT-PCR was used to determine the expression of PD-L1 in NSCLC tissues and adjacent tissues. (f and g) The expressions of PD-L1 in DLBCL cell lines were determined using quantitative RT-PCR. (h) The expression relationship between miR-214 and PD-L1 was evaluated using Spearman’s correlation analysis. **p < 0.01, compared with the NC group; ###p < 0.001, compared with the adjacent tissues; ▲p < 0.05, ▲▲p < 0.01, compared with the NBC group; △△p < 0.01, compared with the OCI-Ly3 cells