Fig. 4
The effect of continuous hypoxia for 3 days on days 1, 3, 5 and 7 of culture with or without a STAT3 inhibitor. a ALP staining on day 7. b Matrix mineralization (alizarin red staining) on day 14. c ALP activity on day 7. d Quantitative analysis of alizarin red staining. e Osteogenic differentiation marker (protein) expression. Data of quantitative analysis are the means ± SD from 5 independent experiments, n = 5. Significant effect of the treatment compared to the control group: *p < 0.05, **p < 0.01 and ***p < 0.001; the CoCl2 group: #p < 0.01, ###p < 0.001; and the CoCl2 + inhibitor group: &&&p < 0.001. Inhibitor: STAT3 inhibitor