Table 1 Primer sequences
Gene | Acc. No | Primer sequence (5′ → 3′) | Size (bp) |
|---|---|---|---|
Hif-1α | NM_176958 | F: GTCCCAGCTACGAAGTTACAGC | 136 |
R: CAGTGCAGGATACACAAGGTTT | Â | ||
Alp | NM_001081082 | F: GGCCATCTAGGACCGGAGA | 79 |
R: TGTCCACGTTGTATGTCTTGG | Â | ||
Opn | NM_001204203 | F: ATCTCACCATTCGGATGAGTCT | 79 |
R: TGTAGGGACGATTGGAGTGAAA | Â | ||
Runx2 | NM_001146038 | F: GACTGTGGTTACCGTCATGGC | 84 |
R: ACTTGGTTTTTCATAACAGCGGA | Â | ||
Col1α1 | NM_007742 | F: GCTCCTCTTAGGGGCCACT | 91 |
R: ATTGGGGACCCTTAGGCCAT | Â | ||
Osx | NM_146065 | F: ATCATTGCAGATCAAACGCCT | 135 |
R: AGCACCCTTTTTCTCATCGTC | Â | ||
Ocn | NM_031368 | F: CACTCCTCGCCCTATTGGC | 112 |
R: CCCTCCTGCTTGGACACAAAG’ |  | ||
Opn | NM_026493 | F: GAAGAGCAAAAAGCGAAACTGG | 153 |
R: TTGGCTGCTTGGTGGAATGT | Â | ||
Vegf | NM_005429 | F: GAGGAGCAGTTACGGTCTGTG | 96 |
R: TCCTTTCCTTAGCTGACACTTGT | Â | ||
Gapdh | NM_008085 | F: AATGGATTTGGACGCATTGGT | 213 |
R: TTTGCACTGGTACGTGTTGAT | Â |