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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: SOX30, a target gene of miR-653-5p, represses the proliferation and invasion of prostate cancer cells through inhibition of Wnt/β-catenin signaling

Fig. 2

SOX30 overexpression repressed prostate cancer cell proliferation and invasion. a PPC-1 and PC-3 cells were transfected with pcDNA3.1/SOX30 vector or empty vector (EV) for 48 h and SOX30 protein expression was examined via western blotting. b The effect of SOX30 overexpression on prostate cancer cell proliferation was determined with the CCK-8 assay after transection of cells with the pcDNA3.1/SOX30 vector for 48 h. c The effect of SOX30 overexpression on prostate cancer cell colony-forming ability was assessed with a colony formation assay. PPC-1 and PC-3 cells were transfected with pcDNA3.1/SOX30 vector for 48 h and then cultured for 14 days to form colonies. d The effect of SOX30 overexpression on prostate cancer cell invasive potential was evaluated using a transwell Matrigel invasion assay. PPC-1 and PC-3 cells were transfected with pcDNA3.1/SOX30 vector for 48 h. The transwell Matrigel invasion assay ran for 24 h. (n = 5, *p < 0.05)

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