Fig. 2From: SOX30, a target gene of miR-653-5p, represses the proliferation and invasion of prostate cancer cells through inhibition of Wnt/β-catenin signalingSOX30 overexpression repressed prostate cancer cell proliferation and invasion. a PPC-1 and PC-3 cells were transfected with pcDNA3.1/SOX30 vector or empty vector (EV) for 48 h and SOX30 protein expression was examined via western blotting. b The effect of SOX30 overexpression on prostate cancer cell proliferation was determined with the CCK-8 assay after transection of cells with the pcDNA3.1/SOX30 vector for 48 h. c The effect of SOX30 overexpression on prostate cancer cell colony-forming ability was assessed with a colony formation assay. PPC-1 and PC-3 cells were transfected with pcDNA3.1/SOX30 vector for 48 h and then cultured for 14 days to form colonies. d The effect of SOX30 overexpression on prostate cancer cell invasive potential was evaluated using a transwell Matrigel invasion assay. PPC-1 and PC-3 cells were transfected with pcDNA3.1/SOX30 vector for 48 h. The transwell Matrigel invasion assay ran for 24 h. (n = 5, *p < 0.05)Back to article page