Fig. 3

SOX30 is an miR-653-5p target gene. a Sequence alignment of the miR-653-5p-binding site within the SOX30 3′-UTR. b MiR-653-5p expression in prostate cancer (n = 495) and normal (n = 52) samples was determined with the starBase Pan-Cancer Analysis Platform. PPC-1, PC-3, LNCaP and DU-145 were the prostate cancer cell lines. The normal prostate epithelial cell line RWPE-1 served as the control. ****p < 0.0001. c The interaction between miR-653-5p and SOX30 3′-UTR was assessed with a dual-luciferase reporter assay using 293 T cells. SOX30 3′-UTR reporter plasmids and miR-653-5p mimics or NC mimics were co-transfected into 293 T cells and incubated for 48 h before determination of luciferase activity. n = 5, *p < 0.05. d and e PPC-1 and PC-3 cells were transfected with miR-653-5p mimics, inhibitor or NC mimics/inhibitor for 48 h. SOX30 mRNA (d) and protein (e) expressions were respectively determined using quantitative real-time PCR and western blot analysis (n = 5, *p < 0.05)