Fig. 6

SOX30 silencing reversed the miR-653-3p inhibition-mediated anti-tumor effect in prostate cancer cells. a PPC-1 and PC-3 cells were co-transfected with miR-653-5p inhibitor and SOX30 siRNA for 48 h. SOX30 protein expression was determined using western blotting. b Cell proliferation was examined with the CCK-8 assay after co-transfection with miR-653-5p inhibitor and SOX30 siRNA for 48 h. c PPC-1 and PC-3 cells were co-transfected with miR-653-5p inhibitor and SOX30 siRNA for 48 h. A transwell Matrigel invasion assay was run for 24 h to determine cell invasion. d Wnt/β-catenin signaling was assessed with a TCF/LEF luciferase reporter assay. PPC-1 and PC-3 cells were co-transfected with TCF/LEF luciferase reporter vector, miR-653-5p inhibitor and SOX30 siRNA and incubated for 48 h before determination of luciferase activity. e, f and g PPC-1 and PC-3 cells were co-transfected with miR-653-5p inhibitor and SOX30 siRNA for 48 h, and relative mRNA expression levels of Axin2 (e), CD44 (f), and c-Myc (g) were determined using quantitative real-time PCR (n = 5, *p < 0.05). h A graphical model of the miR-653-5p–SOX30–Wnt/β-catenin axis in regulating prostate cancer cell proliferation and invasion