Fig. 3

Circ-CAMK2A directly binds to miR-615-5p in LUAD cell lines. a, b Subcellular localization of circ-CAMK2A was determined by qRT-PCR and FISH assays. Nucleus was stained with DAPI. c, d Schematic diagram of circ-CAMK2A with three miR-615-5p binding sites predicted by RegRNA 2.0 online tool. e Luciferase reporter assay of circ-CAMK2A luciferase vector with wild-type or mutated miR-615-5p binding sites in A549 and HCC827 cells after co-transfection with control and miR-615-5p mimics, respectively. (F) RNA pull-down assay using oligo or circ-CAMK2A probe in A549 and HCC827 cells, followed by qRT-PCR analysis for miR-615-5p expression. g, h qRT-PCR analysis for miR-615-5p expression in LUAD tissues and cell lines. i Transwell migration and invasion assays in circ-CAMK2A-overexpressing A549 and circ-CAMK2A-silencing HCC827 cells. j qRT-PCR analysis for circ-CAMK2A and miR-615-5p expression in circ-CAMK2A-overexpressing A549 and circ-CAMK2A-silencing HCC827 cells. k Correlation between circ-CAMK2A and miR-615-5p expression in 58 LUAD tissues, as determined by Pearson’s correlation coefficient. **p < 0.01