Fig. 4

miR-615-5p directly interacts with fibronectin 1 3′-UTR in LUAD cell lines. a Wild-type or mutated miR-615-5p binding sequences on fibronectin 1 3′-UTR. b-c Luciferase reporter assay of fibronectin 1 3′-UTR luciferase vector with wild-type or mutated miR-615-5p binding sites in A549 (b) and HCC827 (c) cells after co-transfection with control and miR-615-5p mimics, respectively. (D) RNA pull-down assay in A549 and HCC827 cells transfected with biotin-labeled control or miR-615-5p mimics, followed by qRT-PCR analysis for fibronectin 1 expression. e qRT-PCR analysis for fibronectin 1 expression in 58 pairs of LUAD and adjacent non-cancerous tissues. f qRT-PCR analysis for fibronectin 1 expression in miR-615-5p-silencing A549 and miR-615-5p-overexpressing HCC827 cells. g-h Western blotting analysis for protein expression of fibronectin 1, MMP2, and MMP9 in miR-615-5p-silencing A549 (G) and miR-615-5p-overexpressing HCC827 (H) cells. GAPDH as an internal reference control. **p < 0.01