Fig. 4

MiR-15 directly targeted NLRX1 to suppress its expression. a – The predicted miR-15 binding site in the 3′-UTR of NLRX1. b and c – MiR-15 mimic, mimic-NC, miR-15 inhibitor or inhibitor-NC were co-transfected into H9c2 cells with NLRX1 3′-UTR (WT) or NLRX1 3′-UTR mut (MUT) reporter plasmids, along with Renilla luciferase pRL-TK plasmids. Luciferase activity was analyzed 48 h post-transfection and normalized to the Renilla luciferase activity. d – The protein expression of NLRX1 in H9c2 cells stably transfected with mimic or inhibitor was determined using western blotting with corresponding histogram analysis, normalizing to GAPDH expression. *p < 0.05