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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Inhibition of microRNA-15 protects H9c2 cells against CVB3-induced myocardial injury by targeting NLRX1 to regulate the NLRP3 inflammasome

Fig. 5

Inhibition of NLRX1 partly reversed the effects of miR-15 inhibition on NLRP3 inflammasome activation induced by CVB3. H9c2 cells were co-transfected with si-NLRX1 or si-NC with miR-15 inhibitor into H9c2 cells for 24 h, followed by CVB3 infection for another 24 h. a – The protein expression of NLRX1 was determined using western blotting with corresponding histogram analysis, normalizing to GAPDH expression. b through d – Production of IL-1β (b), IL-6 (c) and IL-18 (d) was measured using ELISA. e through g – The protein expressions of NLRP3, caspase-1 p20 and pro-caspase-1 were determined using western blotting with corresponding histogram analysis, normalizing to GAPDH expression. h – Caspase 1 activity was determined using a Caspase 1 Activity Assay Kit. *p < 0.05 versus the control group, #p < 0.05 versus the CVB3 group, and &p < 0.05 versus the miR-15 inhibitor+CVB3 group

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