Fig. 5

GNAS promotes LPS-induced HCC cell growth and invasion by interacting with STAT3. a Table listing the GNAS-interacting proteins that coimmunoprecipitated with anti-GNAS antibody from HepG2 cells identified by mass spectrometry. b The interactions of endogenous GNAS and endogenous STAT3 in HepG2 cells were detected by CO-IP. c HepG2 cells were transfected with pCMV-myc-GNAS and pCMV-flag-STAT3 for 24 h, and then the interactions of exogenous GNAS and exogenous STAT3 were detected by CO-IP. d and e HepG2 cells were transfected with pCMV-myc-GNAS for 24 h, treated with a specific inhibitor of STAT3, c188–9, for 30 min, and then stimulated with LPS (5 μg/ml) for 12 h. Cell invasive ability was examined by transwell invasion assays (d). Cell proliferation was examined by MTT assays (e). Data are represented as means ± SD (n = 3; ^*represents P < 0.05)