Table 1 Primers used in this study (F: forward primer; R: reverse primer)
Name | Sequence | Â |
|---|---|---|
Primers for GNAS constructs | ||
 GNAS F | 5′-GCCATGGAGGCCCGAATTCGGGTTCGTTGCAACAAATTGAT-3′ | pCMV-Myc-GNAS |
 GNAS R | 5′-GGCCGCGGTACCTCGAGGTTCGTTGCAACAAATT-3′ | |
Primers for qRT-PCR | ||
 GNAS F | 5′-TGCCTCGGGAACAGTAAGAC-3′ |  |
 GNAS R | 5′-GCCGCCCTCTCCATTAAAC-3′ |  |
 IL-6 F | 5′-ACTCACCTCTTCAGAACGAATTG-3′ |  |
 IL-6 R | 5′-CCATCTTTGGAAGGTTCAGGTTG-3′ |  |
 TNFα F | 5′-CCTCTCTCTAATCAGCCCTCTG-3′ |  |
 TNFα R | 5′-GAGGACCTGGGAGTAGATGAG-3′ |  |
 IL-1β F | 5′-ATGATGGCTTATTACAGTGGCAA-3′ |  |
 IL-1β R | 5′-GTCGGAGATTCGTAGCTGGA-3′ |  |
 IL-8 F | 5′-TTTTGCCAAGGAGTGCTAAAGA-3′ |  |
 IL-8 R | 5′-AACCCTCTGCACCCAGTTTTC-3′ |  |
 IL-10 F | 5′-CCTCCGTCTGTGTGGTTTGAA-3′ |  |
 IL-10 R | 5′-CACTGCGGTAAGGTCATAGGA-3′ |  |
 Bcl-xl F | 5′-GAGCTGGTGGTTGACTTTCTC-3′ |  |
 Bcl-xl R | 5′-TCCATCTCCGATTCAGTCCCT-3′ |  |
 Cyclin D F | 5′-GCTGCGAAGTGGAAACCATC-3′ |  |
 Cyclin D R | 5′-CCTCCTTCTGCACACATTTGAA-3′ |  |
 Mcl1 F | 5′-TGCTTCGGAAACTGGACATCA-3′ |  |
 Mcl1 R | 5′-TAGCCACAAAGGCACCAAAAG-3′ |  |
 GAPDH F | 5′-TCAACAGCAACTCCCACTCTTCCA-3′ |  |
 GAPDH R | 5′-ACCCTGTTGCTGTAGCCGTATTCA-3′ |  |
Sequences of siRNAs | ||
 si-GNAS | 5′-TCGAAGATTGAGGACTACTTTCC-3′ |  |
 si-YTHDF1 | 5′-CCUACGGACAGCUCAGUAAT −3′ |  |
 scrambled siRNA | 5′-UUCUCCGAACGUGUCACGUTT-3′ |  |
Primers used for ChIP | ||
 IL-6 promoter F | 5′-GCTGGCTAGCCTGCTTATGTCAG-3′ |  |
 IL-6 promoter R | 5′-TCATTGAGGCTAGCGCTAAGAA-3′ |  |