Fig. 3

Inhibition of miR-711 reduced oxidative stress-mediated mitochondrial damage. a Treatment with H₂O₂ and CoCl₂ decreased the MMP. Cells were treated with H₂O₂ and CoCl₂ as described in Fig. 1a. Mitochondrial damage was assessed by measuring the MMP by staining cells with green fluorescent DiOC6, followed by flow cytometry. b The effect of treatment duration on the MMP. Cells were treated with 300 μM H₂O₂ and 500 μM CoCl₂ for the indicated hours, respectively. The MMPs were detected as in (a). c AA and H/R decreased the MMP. Cells were treated 20 μM AA or exposed to cold H/R. The MMPs were detected as in (a). d Over-expression of miR-711 increased mitochondrial damage. H9c2 cells were transfected with 1 μg of miR-711 mimic or inhibitors for 48 h prior to H/R. 24 h after reoxygenation, the MMPs were measured by flow cytometry. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. a: treatment groups compared with cells cultured in DMEM-CM; b: treatment with 500 μM CoCl₂ for 12 h compared with treatment for 6 h; c: Treatment with 500 μM CoCl₂ for 24 h compared with treatment for 12 h