Fig. 5
miR-711 was regulated by HIF-1α. a HIF-1α overexpressed by AA, H2O2 and CoCl2. Cells were treated with 20 μM AA, 300 μM H2O2, and 500 μM CoCl2. HIF-1 α was measured by qRT-PCR. b HIF-1α siRNA knocked down HIF-1α. Cells were transfected with HIF-1α siRNA. 48 h after transfection, HIF-1α expression was detected by qRT-PCR (left). Some of the transfected cells were further treated with 300 μM H2O2, and 500 μM CoCl2 for 24 h and HIF-1α expression was measured 24 h after treatment by qRT-PCR (right). c Knockdown of HIF-1α decreased miR-711 expression. MiR-711 expression was detected by qRT-PCR in the cells from (b). d HIF-1 siRNA reduced cell death induced by AA. Cells were transfected with HIF-1 siRNA for 48 h, treated with 20 μM AA for 3 h. AA was replaced with DMEM-CM containing green fluorescent DNA bound dye SYTOX and cells were placed in an IncuCyte system for real time monitoring cell death. Right: representative images of cells collected by the IncuCyte system. Dead cells appeared in green; Left: the percentage of green dead cells in total cells