Fig. 4
From: Blockage of O-linked GlcNAcylation induces AMPK-dependent autophagy in bladder cancer cells
O-GlcNAc negatively regulates phosphorylation of AMPK and ULK1 in 5637 cells. a through d – Whole cell extracts were isolated from 5637 cells treated with DON or TG. Western blotting was used to determine the expression of the proteins. a and b – 5637-GFP-LC3 cells were treated with different doses of DON for 16 h (a) or with DON (50 μM) for different time points (b). c and d – The 5637-GFP-LC3 cells were treated with different doses of TG for 16 h (c) or with TG (10 μM) for different time durations (d). e – The expressions of proteins in 5637-GFP-LC3 cells with knockdown of OGT or OGA, and in the 5637 negative control