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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Blockage of O-linked GlcNAcylation induces AMPK-dependent autophagy in bladder cancer cells

Fig. 5

AMPK is required for O-GlcNAc-mediated regulation of autophagy. a – The expressions of O-GlcNAc (RL2), p-AMPKα (T172), p-ACC (S79), ULK1, p-ULK1(S555), LC3 and p62 in 5637 cells with AMPKα manipulations were measured using western blotting. b – 5637 cells with sh-AMPKα were treated with DON (50 μM) or TG (10 μM) for 16 h, and the expressions of the proteins listed above were measured using western blotting. GAPDH served as an internal control

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