Fig. 6
From: Blockage of O-linked GlcNAcylation induces AMPK-dependent autophagy in bladder cancer cells
AMPK is O-GlcNAcylated. Co-IP was used to test the relationship between O-GlcNAc and AMPK. a – The 5637-GFP-AMPKα cells were treated with TG and cell extracts were used for the Co-IP with an anti-GFP antibody. IP products were subjected to detect the presence of O-GlcNAc (using western blotting with RL2 antibody) and that of AMPKα fused with GFP (using AMPKα antibody). b – The 5637 cells were transfected with an empty vector (Ctrl) or OGT-overexpression (OGT-OE) construct, and then the extracts were used for the Co-IP with an anti-GFP antibody and further analyzed using western blotting with RL2 antibody (left panel) or GFP and HA antibody (right panel)