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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: RETRACTED ARTICLE: Downregulation of miR-483-5p decreases hypoxia-induced injury in human cardiomyocytes by targeting MAPK3

Fig. 5

Restoration of MAPK3 imitated the effects of miR-483-5p inhibitor in hypoxic cardiomyocytes. AC16 cells were transfected with pcDNA3.1-MAPK3 or pcDNA3.1, followed by 24 h hypoxia treatment. a The protein expression of MAPK3 was measured using western blot analysis. b Cell viability was measured using a CCK-8 assay. c and d Cell apoptosis was analyzed using flow cytometry with double Annexin V FITC-PI staining. e through g The content of MDA, the activities SOD and CAT (G) were measured using commercial assay kits. Data are indicated as means ± SD of three independent experiments. **p < 0.01, ***p < 0.001, as compared with pcDNA3.1

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