Fig. 4
CASC2 suppresses hypoxia-induced PASMC proliferation and migration by regulating miR-222 expression. a Expression of miR-222 was detected using qRT-PCR in PASMCs following O2 exposure (21, 10, 3 and 1%) for 24 h. b MiR-222 expression in PASMCs exposed to 3% O2 for 0 h, 12 h, 24 h, 36 h and 48 h was measured by qRT-PCR. Experiments were performed in triplicate. c Expression of miR-222 was detected by qRT-PCR in hypoxia-induced PASMCs transfected with si-NC, si-CASC2, si-CASC2 + anti-miR-NC or si-CASC2 + anti-miR-222 three times. d CCK-8 assay was used to determine the proliferation of hypoxia-induced PASMCs; experiments were conducted at least three times. e Migration ability of hypoxia-induced PASMCs was measured using wound healing assay; bar charts represent relative migrated cells from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001