Fig. 2

MiR-206 suppresses proliferation, migration and invasion of A549 cells. a MiR-206 mimic treatment increased miR-206 mRNA level. b MiR-206 represses proliferation of A549 cells. c and d MiR-206 inhibits horizontal migration of A549 cells. The effect of miR-206 on the horizontal migration of A549 cells was evaluated by wound healing assay. Representative images (100× magnification) and the quantitative data are shown in c and d, respectively. e and f MiR-206 represses the migration and invasion of A549 cells. Representative images (100× magnification) of the transwell migration assay and transwell invasion assay are shown in e. Quantitative data are shown in f. The data were analyzed with GraphPad Prism 5.0 and presented as mean ± SEM, n = 3. ***P < 0.001 compared with the control group. g. MiR-206 downregulates expression of EMT markers. The A549 cells were treated with miR-206 mimic or NC mimic for 24 h, then the cells were collected and subjected to western blotting assay to detect EMT expression. β-actin was set as a loading control