Fig. 5From: TCF 4 tumor suppressor: a molecular target in the prognosis of sporadic colorectal cancer in humansa Western blot analysis of TCF-4 protein. Western blot analysis of TCF-4 protein in normal, adjoining and tumor tissues from CRC Patients. Bar diagram showing densitometric analysis of the TCF-4 protein normalized with β-actin protein in normal, adjoining and tumor tissues. Protein levels of TCF-4 were lower in adjoining and tumor tissues of CRC patients compared to normal tissue. Western blot of β-actin as a loading control. ***p < 0.001 compared to normal tissue. A total of 20 cases each of tumor, adjoining and normal mucosa were analyzed for protein expression by western blotting studies. b Patient survival evaluation. Kaplan-Meier survival curves were constructed and log rank analysis revealed that TCF-4 exon 1 gene mutations had no correlation with overall survival or disease-free survival of the patients (p > 0.05)Back to article page