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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Targeting the 4-1BB costimulatory molecule through single chain antibodies promotes the human T-cell response

Fig. 1

Schematics describing the isolation of human T cells, selection of anti-4-1BB single chain fragment variable antibodies (scFvs), and T-cell treatment. a Isolation of CD3+ T cells from peripheral blood via ficoll and negative selection (depletion of unwanted cells) using magnetic affinity cell sorting (MACS). b Isolation and characterization of four anti-4-1BB scFvs (PI.12, PI.42, PII.16 and PII.29) via phage display technology. c Stimulation of T cells with IL-2 and anti-CD3, treatment with anti-4-1BB scFvs and analysis of the effects of scFvs on T cells

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