Fig. 3

The interaction between miR-637 and IGF-2. a – TargetScan database prediction of the binding sites between IGF-2 and miR-637. b – The targeted relationship between miR-637 and IGF-2 was verified using a dual luciferase reporter gene assay. c and d – The expressions of miR-637 and IGF-2 mRNA showed different trends depending on the ox-LDL concentrations and treatment time. e – The expression of IGF-2 mRNA was determined using real-time PCR after treatment with miR-637 mimics, 100 mg/l ox-LDL or miR-637 mimics+ 100 mg/l ox-LDL. f – The expression of IGF-2 mRNA was determined using real-time PCR after treatment with miR-637 inhibitors, 100 mg/l ox-LDL or miR-637 inhibitors+ 100 mg/l ox-LDL. g – The expression of IGF-2 was determined using western blotting after treatment with miR-637 mimics, 100 mg/l ox-LDL or miR-637 mimics+ 100 mg/l ox-LDL. h – The expression level of IGF-2 was determined using western blotting after treatment with miR-637 inhibitors, 100 mg/l ox-LDL or miR-637 inhibitors+ 100 mg/l ox-LDL. *, ** and *** respectively indicate p < 0.05, p < 0.01 and p < 0.001