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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Downregulation of miR-637 promotes vascular smooth muscle cell proliferation and migration via regulation of insulin-like growth factor-2

Fig. 4

IGF-2 reverses the effect of miR-637 on the proliferation, migration and apoptosis of VSMCs. a and b – real-time PCR was carried out to determine the expressions of miR-637 (a) and IGF-2 mRNA (b) in VSMCs after transfection with miR-637 mimics, pcDNC3.1-IGF-2 or miR-637 mimics +pcDNA3.1-IGF-2. c – Flow cytometry was carried out to monitor the apoptosis of VSMCs in each group. d – A CCK8 assay was done to determine the proliferation of VSMCs in each group. e – A Transwell assay was performed to determine the migration of VSMCs in each group. *, **, *** respectively indicate p < 0.05, p < 0.01, p < 0.001. In Fig. D, * indicates p < 0.05, NC group VS miR-637 mimics group; ## indicates p < 0.01, pcDNA3.1-IGF-2 group VS NC group; & indicates p < 0.05, pcDNA3.1-IGF-2 group VS miR-637 + pcDNA-IGF-2 group

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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