Fig. 1
From: Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
Interaction of HPV16 E6 and Daxx in C33A cells. A Binding of HPV16 E6 and Daxx assessed using western blotting. Photo shows representative blots for: (a) the cell lytic supernatant with anti-Daxx antibody (positive control for Daxx); (b) the complex of Daxx pulled down by the anti-Daxx antibody with anti-Daxx antibody; (c) the complex of Daxx pulled down by the anti-E6 antibody with anti-Daxx antibody; (d) the complex pulled down by the IgG antibody with anti-Daxx antibody (negative control of Daxx); (e) the cell lytic supernatant with anti-E6 antibody (positive control of HPV16 E6); (f) the complex pulled down by the anti-Daxx antibody with anti-E6 antibody; (g) the complex pulled down by the anti-E6 antibody with anti-E6 antibody; (h) the complex pulled down by the IgG antibody with anti-E6 antibody (negative control of HPV16 E6). B Localization of HPV16 E6 and Daxx via indirect immunofluorescence. (a) C33A cells with mouse anti-E6 antibodies visualized with TRITC-conjugated goat anti-mouse IgG (red); (b) C33A cells with rabbit anti-Daxx antibodies visualized with FITC -conjugated goat anti-rabbit IgG (green); (c) C33A cells with DNA dye (blue); (d) a and c overlapped; (e) b and c overlapped; (f) a and b overlapped, showing yellow fluorescence; (h) a, b and c overlapped