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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells

Fig. 2

Effects of HPV16 E6 on Daxx expression. A Relative quantification of Daxx RNA via quantitative PCR. Relative quantification of Daxx was evaluated using the double delta Ct (ΔΔCt) method. , p < 0.05. B Daxx protein expression evaluated via western blotting. (a) C33A cells without transfection; (b) C33A cells with empty vector transfection; (c) C33A cells with Daxx transfection; (d) C33A cells with E6 and Daxx co-transfection; (e) C33A cells with E6 transfection. C Integrated density values based on western blotting results. IntDen TP/IR describes the ratio of the integrated density of the internal reference to that of the target protein (IntDen IR/TP). , p < 0.01

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