Fig. 2

Effects of HPV16 E6 on Daxx expression. A Relative quantification of Daxx RNA via quantitative PCR. Relative quantification of Daxx was evaluated using the double delta Ct (ΔΔCt) method. ★, p < 0.05. B Daxx protein expression evaluated via western blotting. (a) C33A cells without transfection; (b) C33A cells with empty vector transfection; (c) C33A cells with Daxx transfection; (d) C33A cells with E6 and Daxx co-transfection; (e) C33A cells with E6 transfection. C Integrated density values based on western blotting results. IntDen TP/IR describes the ratio of the integrated density of the internal reference to that of the target protein (IntDen IR/TP). ★★, p < 0.01