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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276

Fig. 1

Nutlin-3 stabilizes p53-MDM2 and HSP70-MDM2 protein-protein interactions in situ. a, c Proximity ligation assay of A375 cells treated with DMSO or 10 μM Nutlin-3 for 24 h. Green fluorescence spots suggest that both target proteins (p53-MDM2 or HSP70-MDM2) are within interacting range. The cells were counterstained with DAPI (blue). b, d The quantification of p53-MDM2 or HSP70-MDM2 interaction after incubation with DMSO and Nutlin-3. The results represent the mean ± SD of technical triplicates. Statistical analysis was performed by one-way ANOVA with a Bonferroni post hoc test, ** p < 0.01

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