Fig. 6

Activity of CD4+ T-cells as a function of ATG5 genotype. Representative flow cytometric histograms with the percentage of proliferating CD4 + T-cells (indicated with black bar) for a A549-Atg5-wt, b A549-Atg5-null, c A549-Atg5-resc, d A549-Atg5-null cells in the presence of PD-L1 blocking antibody, e only T-cells as negative control and f T-cells stimulated with CD/CD28 as positive control. T-cells were labelled with CFSE and co-cultured with the panel of A549 cells at the T-cell: A549 ratio of 1:5. Results represent the mean ± SD of technical triplicates, each with 30,000 counted cells. g Normalized quantitative analysis of proliferating T-cells derived from FACS on a-f. Statistical analysis was performed by one-way ANOVA with Bonferroni post hoc test, ** p < 0.01, *** p < 0.001