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Fig. 7 | Cellular & Molecular Biology Letters

Fig. 7

From: The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276

Fig. 7

Effects of CD276 depletion on cell cycle parameters. HCT116 p53-wt were transfected with siRNA towards CD276 (CD276si) for 48 h. a Detection of CD276 and β-actin on Western blot at molecular weights of 60 and 42 kDa, respectively, NTC – non-targeting control. b Flow cytometry analysis of cell cycle parameters. Distribution of G1 and G2 phases in HCT116 cells with normal and depleted CD276 protein level (CD276si) and after treatment with 20 μM Nutlin-3 or untreated for 48 h. Results represent the mean ± SD of technical triplicates, each with 10,000 counted cells. c Representative histograms of cell cycle after staining with propidium iodide

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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