Fig. 2
miR-361-3p overexpression suppresses serotonin-induced cell proliferation of hPASMCs. hPASMCs were divided into four groups: blank (no treatment), serotonin (treated with 250 μmol/l serotonin for 48 h), serotonin + miR-NC (after transfection with miR-NC for 24 h, treated with 250 μmol/l serotonin for 48 h), and serotonin + miR-361-3p group (after transfection with miR-361-3p mimic for 24 h, treated with 250 μmol/l serotonin for 48 h). a Results of MTS assay showing the OD490nm of the four groups. b Results of EdU staining showing the percentage of EdU-positive cells. c Representative images of EdU staining from the four groups. d Graphical representation of the percentage of cells in the G1-, S- and G2-phases. e Representative images of the cell cycle assay. f Cyclin D1, cyclin E1, cleaved caspase-9 and Bcl-2 levels were determined using western blot analysis. *p < 0.05