Fig. 3

SERT is a target of miR-361-3p. a The binding site of miR-361-3p in the 3′-UTR of SERT. b Results of luciferase reporter assay. 293 T cells were co-transfected with wild type 3′-UTR plasmid and miR-361-3p mimic, wild type 3′-UTR plasmid with miR-NC, mutant 3′-UTR plasmid with miR-361-3p mimic, or mutant 3′-UTR plasmid with miR-NC. After transfection for 48 h, the activities of firefly and Renilla luciferase were measured and relative luciferase activity is expressed as the ratio of Renilla luciferase to firefly luciferase (R/F). c SERT mRNA level in RIP products analyzed using quantitative RT-PCR. The cell lysate before immunoprecipitation was termed the ‘input’ group. The products of a RIP assay targeting AGO2 or IgG were named the AGO2 group or IgG group. d SERT level in blank (no treatment), serotonin (treated with 250 μmol/l serotonin for 48 h), serotonin + miR-NC (after transfection with miR-NC for 24 h, treated with 250 μmol/l serotonin for 48 h), and serotonin + miR-361-3p (after transfection with miR-361-3p mimic for 24 h, treated with 250 μmol/l serotonin for 48 h) groups. *p < 0.05