Fig. 4
SERT overexpression alleviates the effect of miR-361-3p overexpression on serotonin-induced cell proliferation of PASMCs. serotonin treated-hPASMCs were divided into three groups: miR-NC + pcDNA (transfected with miR-NC and empty pcDNA3.1 + plasmid), miR-361-3p + pcDNA (transfected with miR-361-3p mimic and empty pcDNA3.1 + plasmid), and miR-361-3p + ov-SERT (transfected with miR-361-3p mimic and ov-SERT). a Results of the MTS assay showing the OD490nm for the three groups. Results of EdU staining with the percentage of EdU-positive cells (b) and representative images of EdU staining from the three groups (c). d Results of the cell cycle assay with representative images from the three groups (d) and a graphical representation of the percentage of cells at the G1-, S- and G2-phases (e). f Level of SERT, cyclin D1 and cyclin E1 expressions determined using western blot analysis. GAPDH served as the loading control. *p < 0.05