Fig. 1

Effect of W8B2⁺ CSCs in vitro differentiation on cardiac markers. a Transcript coding for cardiac transcription factors analyzed by TLDA in differentiated vs undifferentiated W8B2⁺ CSCs. b Determination by immunostaining of protein expression profile of GATA4, MEF2C and NKX 2.5 (b1) and troponin T, a-actinin and b-MHC (b2) after in vitro differentiation of W8B2⁺ CSCs. Nuclei are represented in blue with TOPRO staining. Scale bar 100 μm. c Transcript coding for cardiac contractile structures analyzed by TLDA in differentiated vs undifferentiated W8B2⁺ CSCs. d Transcript coding for natriuretic peptides [atrial (ANP) and brain (BNP)] analyzed by TLDA in differentiated vs undifferentiated W8B2⁺ CSCs. The graphs illustrate the gene expression variations (expressed in log 2) of the differentiated cells versus the undifferentiated cells. Values between 1 and − 1 (horizontal red lines) indicate that gene expression does not vary (this corresponds to variations of + 2 times and − 2 times compared to undifferentiated cells). Each bar represents the mean value ± S.E.M. (standard error of the mean). (N = 4 patients)