Fig. 4
Role of L-type calcium channel and NCX exchanger activity in W8B2+ CSCs after 28 days of in vitro differentiation. a Effect of 5 µM nifedipine (L-type CaV inhibitor) and c 3 µM SEA-0400 (NCX exchanger inhibitor) on spontaneous calcium activity recorded in differentiated CSCs. Graphs illustrate variations in [Ca2+]i as represented by changes in the ΔF/F0 ratio. ΔF = F − F0 (F: fluorescence emitted by CaMP at a given time; F0: basal fluorescence emitted by GCaMP). Bar graphs (1), (2), (3) represent the evolution of the peak amplitude, the duration and frequency of calcium oscillations, respectively, recorded from differentiated W8B2+ CSCs after treatment with nifedipine (5 µM) (b) and SEA (3 µM) (d). (N = 3 patients, n: represents the number of events (oscillations) recorded, AU: arbitrary unit, ns: not significant). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control; t-test paired followed by a Wilcoxon test