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Table 1 List of primers used for qRT-PCR reactions

From: Differentiation of two human neuroblastoma cell lines alters SV2 expression patterns

Target Forward primer Reverse primer Annealing temp (°C)
RPL13A* 5ʹ-cctggaggagaagaggaaagaga-3ʹ 5ʹ-ttgaggacctctgtgtatttgtcaa-3ʹ 57.1
RPL19* 5ʹ-tgaggagaatgaggattt-3ʹ 5ʹ-gtacaggctgtgatacat-3ʹ 54.1
H3F3B* 5ʹ-cattatctaggtccttgta-3ʹ 5ʹ-aatacagcactattatgg-3ʹ 54.1
B2M* 5ʹ-gactggtctttctatctct-3ʹ 5ʹ-cttcaaacctccatgatg-3ʹ 55
HPRT1* 5ʹ-aagcctaagatgagagtt-3ʹ 5ʹ-ccacagaactagaacattg-3ʹ 55.8
GAPDH* 5ʹ-cctcaagatcatcagcaat-3ʹ 5ʹ-ttccacgataccaaagtt-3ʹ 55
TUBB3* 5ʹ-ggcatctcttgagaacaa-3ʹ 5ʹ-gacctgtacctgtctctc-3ʹ 55
SV2A 5ʹ-agcatgacgaaggtgaat-3ʹ 5ʹ-gtagctgtgacgtgttgta-3ʹ 58.9
SV2B 5ʹ-ttcaggcactaaggtctct-3ʹ 5ʹ-aaggaagcgattctcaatgtt-3ʹ 57.1
SV2C 5ʹ-ctggagcgttctgttttg-3ʹ 5ʹ-aattttcacagcctcctttc-3ʹ 57.1
DAT 5ʹ-gccgtggtcttcttcatcat-3ʹ 5ʹ-aacagggacaggaggaaggt-3ʹ 58.1
CHAT 5ʹ-agatgttctgctgctatg-3ʹ 5ʹ-gaaaaggatggtctctgg-3ʹ 55.8
NET 5ʹ-agcttttggtggtcagatgg-3ʹ 5ʹ-cagagcaagagcagcatgag-3ʹ 61.4
SNAP25 5ʹ-taacacttcttacgcaatg-3ʹ 5ʹ-gaacgaaccaactgatt-3ʹ 57.1
FGFR3 5ʹ-agactgaaattacgggta-3ʹ 5ʹ-ccatatacacagcatctatt-3ʹ 55.8
  1. Primers used for housekeeping genes are marked with an asterisk (*)