Table 1 List of primers used for qRT-PCR reactions
From: Differentiation of two human neuroblastoma cell lines alters SV2 expression patterns
Target | Forward primer | Reverse primer | Annealing temp (Ā°C) |
|---|---|---|---|
RPL13A* | 5Ź¹-cctggaggagaagaggaaagaga-3Ź¹ | 5Ź¹-ttgaggacctctgtgtatttgtcaa-3Ź¹ | 57.1 |
RPL19* | 5Ź¹-tgaggagaatgaggattt-3Ź¹ | 5Ź¹-gtacaggctgtgatacat-3Ź¹ | 54.1 |
H3F3B* | 5Ź¹-cattatctaggtccttgta-3Ź¹ | 5Ź¹-aatacagcactattatgg-3Ź¹ | 54.1 |
B2M* | 5Ź¹-gactggtctttctatctct-3Ź¹ | 5Ź¹-cttcaaacctccatgatg-3Ź¹ | 55 |
HPRT1* | 5Ź¹-aagcctaagatgagagtt-3Ź¹ | 5Ź¹-ccacagaactagaacattg-3Ź¹ | 55.8 |
GAPDH* | 5Ź¹-cctcaagatcatcagcaat-3Ź¹ | 5Ź¹-ttccacgataccaaagtt-3Ź¹ | 55 |
TUBB3* | 5Ź¹-ggcatctcttgagaacaa-3Ź¹ | 5Ź¹-gacctgtacctgtctctc-3Ź¹ | 55 |
SV2A | 5Ź¹-agcatgacgaaggtgaat-3Ź¹ | 5Ź¹-gtagctgtgacgtgttgta-3Ź¹ | 58.9 |
SV2B | 5Ź¹-ttcaggcactaaggtctct-3Ź¹ | 5Ź¹-aaggaagcgattctcaatgtt-3Ź¹ | 57.1 |
SV2C | 5Ź¹-ctggagcgttctgttttg-3Ź¹ | 5Ź¹-aattttcacagcctcctttc-3Ź¹ | 57.1 |
DAT | 5Ź¹-gccgtggtcttcttcatcat-3Ź¹ | 5Ź¹-aacagggacaggaggaaggt-3Ź¹ | 58.1 |
CHAT | 5Ź¹-agatgttctgctgctatg-3Ź¹ | 5Ź¹-gaaaaggatggtctctgg-3Ź¹ | 55.8 |
NET | 5Ź¹-agcttttggtggtcagatgg-3Ź¹ | 5Ź¹-cagagcaagagcagcatgag-3Ź¹ | 61.4 |
SNAP25 | 5Ź¹-taacacttcttacgcaatg-3Ź¹ | 5Ź¹-gaacgaaccaactgatt-3Ź¹ | 57.1 |
FGFR3 | 5Ź¹-agactgaaattacgggta-3Ź¹ | 5Ź¹-ccatatacacagcatctatt-3Ź¹ | 55.8 |