Fig. 4

mROS mediates iron overload-induced autophagy in bone marrow mononuclear cells. a—Quantification of mROS levels using a fluorescence spectrometer after bone marrow mononuclear cells were treated with FAC at different concentrations for 24 h. b through d—Bone marrow mononuclear cells were preincubated with Mito-TEMPO (10 mM) for 2 h and then treated with 200 μM FAC, then the mROS levels (b), LC3 levels (c) and cell viability (d) were determined. The values are presented as the means ± SEM, **p < 0.05 vs. the control group, ^##p < 0.05 vs. the FAC group