Fig. 5

Iron overload can increase acetylated-SOD2 expression and decrease SIRT3 protein expression and activity in a dose-dependent manner. a—SOD2 activity in bone marrow mononuclear cells treated with FAC (μM). b—Representative immunoblot of SOD2 and SIRT3 protein levels in bone marrow mononuclear cells. β-actin was used as an internal standard for protein loading. c—Acetylation of SOD2 after FAC exposure was determined via immunoprecipitation with an anti-SOD2 antibody, followed by immunoblot analysis of acetylated-lysine. β-actin was used as an internal standard for protein loading. d—Quantitative real-time PCR analysis was applied to determine SIRT3 mRNA levels. e—SIRT3 activity was measured based on an enzymatic reaction using a SIRT3 assay kit. The values are presented as the means ± SEM, **p < 0.05 vs. the control group