Fig. 6

SIRT3–SOD2 modulates iron overload-induced mROS accumulation and autophagy in bone marrow mononuclear cells in vitro. a—SIRT3 overexpression induced ac-SOD2 after 200 μM FAC treatment. b through e—After SIRT3 overexpression, SOD2 activity in bone marrow mononuclear cells (b), mROS production in bone marrow mononuclear cells (c) and cell viability (e) were tested. d—A representative immunoblot of LC3 protein levels in bone marrow mononuclear cells. β-actin was used as an internal standard for protein loading. The values are presented as the means ± SEM, **p < 0.05 vs. the control group, ^##p < 0.05 vs. the FAC group