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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Deficiency of αII-spectrin affects endothelial cell–matrix contact and migration leading to impairment of angiogenesis in vitro

Fig. 2

Knockdown of αII-spectrin is associated with defective cell adhesion and spreading. a Static cell adhesion assay: 72 h after transfection with either non-relevant siRNA (Nr siRNA) or siRNA targeting αII-spectrin (Sp siRNA), HMEC-1 cells were tested for their ability to bind on fibronectin and laminin. The percentage of adherent Sp siRNA-treated cells 2 h after plating is significantly lower than that for control or Nr siRNA-treated cells on both substrates b Kinetic analysis of adhesion of primary HUVECs on laminin. HUVECs expressing GFP-actin were transfected with either Sp siRNA or Nr siRNA. Adhesion on laminin was followed using video microscopy

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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