Fig. 4
From: MicroRNA-429 inhibits neuroblastoma cell proliferation, migration and invasion via the NF-κB pathway
IKKβ was identified as a target gene of miR-429. a – The binding sites between miR-429 and IKKβ. b – The miR-429 mimic decreased the luciferase reporter activity of IKKβ. c – Downregulation of miR-429 expression increased the expression of IKKβ at the mRNA level in SH-SY5Y, SK-N-SH and IMR-32 cells. d – Overexpression of miR-429 inhibited the expression of IKKβ at the mRNA level in SH-SY5Y, SK-N-SH and IMR-32 cells. e – Western blot showing IKKβ protein expression levels in SH-SY5Y, SK-N-SH and IMR-32 cells after transfection with NC-mimic, miR-429 mimics. f – Western blot showing IKKβ protein expression levels in SH-SY5Y, SK-N-SH and IMR-32 cells after transfection with NC-inhibitor and miR-429 inhibitor. GAPDH was used as the endogenous control. Error bars represent the means ± SEM of at least three independent experiments. N.S.: not significant; *p < 0.05 and **p < 0.01 vs. the control group