Skip to main content
Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: ZNF674-AS1 antagonizes miR-423-3p to induce G0/G1 cell cycle arrest in non-small cell lung cancer cells

Fig. 5

ZNF674-AS1 causes a G0/G1 cell cycle arrest through upregulation of p21. a Flow cytometric analysis showed that overexpression of ZNF674-AS1 arrested NSCLC cells at the G0/G1 phase. *P < 0.05 compared to vector-transfected cells. b Western blot analysis of indicated proteins. c ZNF674-AS1 overexpression increased the levels of p21 mRNA in NSCLC cells. *P < 0.05. d Knockdown of p21 in NSCLC cells by transfection of siRNAs. siCtrl: control siRNA; sip21: p21-targeting siRNA. e MTT assay showed that p21 knockdown rescued ZNF674-AS1-mediated growth of A549 cells. f Cell cycle analysis conducted in A549 cells transfected with indicated constructs. *P < 0.05 compared to vector-transfected cells; #P < 0.05 compared to cells transfected with ZNF674-AS1 alone

Back to article page