Fig. 6

ZNF674-AS1 antagonizes miR-423-3p to derepress p21. a Analysis of candidate miRNAs in NSCLC cells transfected with vector or ZNF674-AS1. ^*P < 0.05. ns: no significance. b Analysis of p21 mRNA levels after transfection with indicated constructs. ^*P < 0.05. c Western blot analysis of p21 protein levels in NSCLC cells treated as in (b). d MTT assay showed that ZNF674-AS1-mediated growth suppression was reversed by miR-423-3p. e Cell cycle analysis conducted in NSCLC cells transfected with indicated constructs. ^*P < 0.05 compared to vector-transfected cells; ^#P < 0.05 compared to cells transfected with ZNF674-AS1 alone. f An inverse correlation was detected between expression of ZNF674-AS1 and miR-423-3p in NSCLC specimens (n = 83). g Schematic model of ZNF674-AS1/miR-423-3p/p21 pathway