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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: The NOTCH-HES-1 axis is involved in promoting Th22 cell differentiation

Fig. 4

HES-1 was indispensable in naïve CD4+ T cell differentiation into Th22 cells. Naïve CD4+ T cells were transfected with HES-1-siRNA, HES-1 plasmid, or their controls. Subsequently, the transfection cells were cultured in conditions designed to induce Th22 differentiation. a The intracellular IL-17A and IL-22 in transfection CD4+ T cells were assayed by flow cytometry 6 days after the combination of factors induced Th22 cells. b Quantitative analysis of the percentage of IL-22+ IL-17A cells was performed. c RT-PCR was performed to evaluate the expression of Nicd, Hes-1, Ahr, and Il-22. d Western blot for p-STAT3, STAT3, NICD, Hes-1, AHR, and IL-22 was performed to assess the alteration. ei The relatively quantified protein bands from western blot were executed with the Quantity One software and data were represented as the means ± S.E.M. of three different experiments. *p < 0.05, ***p < 0.001, compared with the control group; #p < 0.5, ##p < 0.01, for HES-1 siRNA versus M-HES-1siRNA groups; p < 0.05, p < 0.01, p < 0.001, for pCMV6-AC-HES-1 versus vector control groups

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