Fig. 5
Ex vivo tissue colocalization studies using fluorescence microscopy. Muscle tissue sections from mouse ischemic hindlimbs at one week after HLi were incubated with 1 µM RAGE-targeted nanoparticles and RAGE antibody conjugated with FITC (a). Studies demonstrated a significant degree of RAGE-targeted probe and antibody colocalization. Images were analyzed using ImageJ’s Coloc2 plugin and Pearson’s r value was calculated (b)