Fig. 5

Actin polymerization can promote localization of YAP protein to the cytoplasm. a Cells were cultured in medium containing different concentrations of JAS. Then, cells were fixed and immunofluorescence stained with anti-YAP antibodies. Fluorescence for YAP and actin is shown in red and green, respectively. Nuclear staining is represented in blue. All images were obtained using a 63 × oil immersion lens on the confocal microscope; b Western blot analysis for YAP and p-YAP (Ser127) of hASCs cultured in medium containing different concentrations of JAS. Scale bar = 20 µm