Fig. 1

Subcellular localization of GAREM1 and GAREM2 in the EGF-stimulated cells. a Schematic representations of GAREM1 (upper) and GAREM2 (lower) primary structure. Tyrosine residues (Y) of phosphorylation and the surrounding amino acid sequence in GAREM1 are indicated by numbers. Proline-rich regions (P-rich) that may bind the SH3 domain are indicated in the box. The amino acid sequence surrounding the phosphorylation site, Y453, is LP_phosphoYEEL; this site is a good match to the consensus sequence of the Shp binding site (ITIM). In addition, CABIT and SAM domains are indicated by underlined text. b Different subcellular localization between GAREM1 and GAREM2 in the NGF-stimulated PC-12 cells. Representative images of each endogenous GAREM1 (left) and GAREM2 (right) in PC-12 cells with (NGF 30 min) or without (NGF 0 min) NGF stimulation. Immunofluorescence staining was performed with specific antibodies against each GAREM subtype. c Different subcellular localization between GAREM1 and GAREM2 in the EGF-stimulated COS-7 cells. Representative images of each GFP-GAREM in COS-7 cells with (EGF 15 min) or without (EGF 0 min) EGF stimulation. Time-lapse images for tracing GFP-GAREM1 (upper), GFP-GAREM2 (lower). COS-7 cells were transfected with each GFP-GAREM after EGF treatment. The images were taken for 15 min at regular intervals of 30 s (Additional file 1: GAREM1, Additional file 2: GAREM2). Scale bars = 10 μm. d Representative images of GFP-GAREM1 (left) and GFP-GAREM2 (right) in COS-7 cells treated with EGF for 45 min