Fig. 2

Identification of a necessary region for forming GAREM2-specific dot-like aggregation by using chimera proteins of both GAREM1 and GAREM2. a Schematics of the GFP-fused full-length and chimera protein constructs of GAREM1 and GAREM2. The numbers indicate amino acid residues. b All recombinant proteins were expressed as the N-terminal GFP-fused form. Each construct was transfected into COS-7 cells, and then we analyzed their localization by GFP-fluorescence signal in the EGF-stimulated cells for 30 min. Representative results are shown. Scale bars = 10 μm. c Confirmation of expressing chimera GAREM proteins in COS-7 cells by immunoblotting using with GFP antibody